
Curriculum for the course:
Flow cytometry - principles, working technique and applications in biomedical research
Lectures
Flow cytometry - general considerations and principle of the technique
Parameters that can be analysed by flow cytometry
Dyes suitable for use on flow cytometers, compensation of fluorescence
Applying gates for subpopulation analysis
Immunophenotyping and stem cell characterization by flow cytometry
Cell cycle analysis by flow cytometry
Apoptosis and cell viability assessment by flow cytometry
Assessing cell proliferation using flow cytometry
Clinical applications of flow cytometry in hematology
Clinical applications of flow cytometry in human and animal reproduction
Cell sorting by flow cytometry, fluorescent proteins, and their application in bioimaging
Evaluation of knowledge
Practical works
Presentation of the flow cytometry laboratory and of the instrument.
Flow cytometer start-up and shutdown. Calibration of the device.
Setting up an experiment and use of flow cytometer software
Immunophenotyping and stem cell characterization by flow cytometry
Cell cycle analysis by flow cytometry
Apoptosis and cell viability assessment by flow cytometry
Assessing cell proliferation using flow cytometry
Clinical applications of flow cytometry in hematology
Clinical applications of flow cytometry in human and animal reproduction