Lectures

Flow cytometry - general considerations and principle of the technique

Parameters that can be analysed by flow cytometry

Dyes suitable for use on flow cytometers, compensation of fluorescence

Applying gates for subpopulation analysis

Immunophenotyping and stem cell characterization by flow cytometry

Cell cycle analysis by flow cytometry

Apoptosis and cell viability assessment by flow cytometry

Assessing cell proliferation using flow cytometry

Clinical applications of flow cytometry in hematology

Clinical applications of flow cytometry in human and animal reproduction

Cell sorting by flow cytometry, fluorescent proteins, and their application in bioimaging

Evaluation of knowledge 

Practical works

Presentation of the flow cytometry laboratory and of the instrument.

Flow cytometer start-up and shutdown. Calibration of the device.

Setting up an experiment and use of flow cytometer software

Immunophenotyping and stem cell characterization by flow cytometry

Cell cycle analysis by flow cytometry

Apoptosis and cell viability assessment by flow cytometry

Assessing cell proliferation using flow cytometry

Clinical applications of flow cytometry in hematology

Clinical applications of flow cytometry in human and animal reproduction